108060004795 methyltransferase family Proteins 0.000 claims abstract description 5.Assuming both NcoI-HF and NotI-HF are supplied at 20,000 U/mL, 0.5 L of each enzyme would result in 1 L total, which is only 2 of the total volume of the reaction. 102000016397 methyltransferase family Human genes 0.000 claims abstract description 5 Adding too much enzyme, particularly if the final volume of combined enzymes is greater than 10 of the overall reaction, can lead to star activity with many enzymes.239000012505 Superdex™ Substances 0.000 claims abstract description 5 A restriction enzyme or restriction endonuclease is defined as a protein that recognizes a specific, short nucleotide sequence and.230000014509 gene expression Effects 0.000 claims abstract description 22 Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering.(Clontech) was inserted into the XhoI-NotI sites of pC. 108090000623 proteins and genes Proteins 0.000 claims abstract description 26 the Cre enzyme is introduced, thus the alterations are referred to as conditional alterations.102000004169 proteins and genes Human genes 0.000 claims abstract description 26 NotI For in vitro use only Unit Definition: One unit is the amount of enzyme required to completely digest 1 g of Adenovirus-2 DNA (7 sites) in 1 hour in a.238000004519 manufacturing process Methods 0.000 title claims abstract description 11.241000588724 Escherichia coli Species 0.000 title claims abstract description 16.108091007521 restriction endonucleases Proteins 0.000 title claims abstract description 39.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Universal Biological Systems (anhui) Co Ltd filed Critical Universal Biological Systems (anhui) Co Ltd Priority to CN201710301236.2A priority Critical patent/CN106967743A/en Publication of CN106967743A publication Critical patent/CN106967743A/en Status Pending legal-status Critical Current Links Thermo Scientific FastDigest NotI restriction enzyme recognizes GCGGCCGC site and cuts best at 37C in 515 minutes using universal FastDigest Buffer. Original Assignee Universal Biological Systems (anhui) Co Ltd Priority date (The priority date is an assumption and is not a legal conclusion. Universal Biological Systems (anhui) Co Ltd The two enzymes used are NotI and any one of the following four enzymes: EcoRV. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) The first step is the double- restriction-enzyme digestion of the BAC DNA. Inventor 李森 雍德祥 邹刚刚 王方平 刘宗文 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Pending Application number CN201710301236.2A Other languages Chinese ( zh)
Google Patents A kind of production method of restructuring NotI restriction enzymes in Escherichia coliĭownload PDF Info Publication number CN106967743A CN106967743A CN201710301236.2A CN201710301236A CN106967743A CN 106967743 A CN106967743 A CN 106967743A CN 201710301236 A CN201710301236 A CN 201710301236A CN 106967743 A CN106967743 A CN 106967743A Authority CN China Prior art keywords noti restriction enzymes methylase restructuring expression Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CN106967743A - A kind of production method of restructuring NotI restriction enzymes in Escherichia coli NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).CN106967743A - A kind of production method of restructuring NotI restriction enzymes in Escherichia coli Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function.
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